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\u6c22\u6c27\u5316\u94a0<\/h3>\n<p>\u56fd\u836f\u6caa\u8bd5\u662f\u4e2d\u56fd\u5316\u5b66\u8bd5\u5242\u9886\u57df\u7684\u56fd\u5bb6\u961f\u54c1\u724c\uff0c\u5386\u53f2\u60a0\u4e45\uff0c\u5e02\u573a\u8986\u76d6\u5e7f\u6cdb\u3002\u8fd9\u6b3eGR\u7ea7\uff08\u4f18\u7ea7\u7eaf\uff09\u6c22\u6c27\u5316\u94a0\u9897\u7c92\uff0c\u7eaf\u5ea6\u8fbe\u5230\u56fd\u6807\u6700\u9ad8\u8bd5\u5242\u7b49\u7ea7\uff0c\u6742\u8d28\u542b\u91cf\u4f4e\uff0c\u6eb6\u89e3\u6027\u597d\u3002\u4ea7\u54c1\u4f18\u52bf\u5728\u4e8e\u51fa\u8272\u7684\u672c\u571f\u5316\u4f9b\u5e94\u94fe\u4fdd\u969c\uff0c\u4f9b\u8d27\u7a33\u5b9a\u53ca\u65f6\uff0c\u4e14\u4ef7\u683c\u76f8\u5bf9\u4e8e\u8fdb\u53e3\u54c1\u724c\u6709\u663e\u8457\u4f18\u52bf\u3002\u5176\u8d28\u91cf\u80fd\u591f\u6ee1\u8db3\u7edd\u5927\u591a\u6570\u9ad8\u6821\u6559\u5b66\u3001\u5e38\u89c4\u68c0\u6d4b\u53ca\u4e00\u822c\u6027\u79d1\u7814\u5b9e\u9a8c\u7684\u9700\u6c42\u3002\u5bf9\u4e8e\u9884\u7b97\u6709\u9650\u4f46\u53c8\u9700\u8981\u53ef\u9760\u8d28\u91cf\u8bd5\u5242\u7684\u9ad8\u6821\u5b9e\u9a8c\u5ba4\u3001\u4f01\u4e1a\u8d28\u68c0\u90e8\u95e8\u53ca\u521d\u521b\u7814\u53d1\u56e2\u961f\u6765\u8bf4\uff0c\u56fd\u836f\u6caa\u8bd5\u7684GR\u7ea7\u4ea7\u54c1\u63d0\u4f9b\u4e86\u6781\u9ad8\u7684\u6027\u4ef7\u6bd4\uff0c\u662f\u517c\u987e\u8d28\u91cf\u4e0e\u6210\u672c\u7684\u52a1\u5b9e\u4e4b\u9009\u3002<\/p>\n<h3>3. 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Tris-HCl\u7f13\u51b2\u76d0<\/h3>\n<p>\u963f\u62c9\u4e01\u54c1\u724c\u5728\u79d1\u7814\u8bd5\u5242\u7535\u5546\u6e20\u9053\u8868\u73b0\u975e\u5e38\u6d3b\u8dc3\uff0c\u4ee5\u4ea7\u54c1\u7ebf\u4e30\u5bcc\u3001\u89c4\u683c\u7075\u6d3b\u8457\u79f0\u3002\u8fd9\u6b3e\u751f\u5316\u7ea7Tris-HCl\u7f13\u51b2\u76d0\u9884\u6df7\u7c89\u672b\uff0c\u7ecf\u8fc7\u4e25\u683c\u7684\u65e0DNA\u9176\u3001\u65e0RNA\u9176\u3001\u65e0\u86cb\u767d\u9176\u5904\u7406\uff0c\u4e13\u4e3a\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u8bbe\u8ba1\u3002\u5176\u6838\u5fc3\u4f18\u52bf\u5728\u4e8e\u7cbe\u51c6\u7684\u573a\u666f\u5316\u5b9a\u4f4d\u548c\u4fbf\u6377\u6027\uff0c\u7528\u6237\u65e0\u9700\u81ea\u884c\u79f0\u91cf\u914d\u5236\uff0c\u6eb6\u89e3\u540e\u5373\u53ef\u83b7\u5f97pH\u503c\u7cbe\u786e\u7684\u7f13\u51b2\u6db2\uff0c\u5927\u5927\u8282\u7701\u4e86\u5b9e\u9a8c\u51c6\u5907\u65f6\u95f4\u5e76\u51cf\u5c11\u4e86\u79f0\u91cf\u8bef\u5dee\u3002\u5728\u751f\u547d\u79d1\u5b66\u3001\u57fa\u56e0\u5de5\u7a0b\u7b49\u9886\u57df\u7684\u65e5\u5e38\u5b9e\u9a8c\u4e2d\uff0c\u963f\u62c9\u4e01\u7684\u8fd9\u7c7b\u4e13\u7528\u8bd5\u5242\u51ed\u501f\u5176\u4f18\u5f02\u7684\u9002\u7528\u6027\u548c\u5408\u7406\u7684\u4ef7\u683c\uff0c\u6df1\u53d7\u5e7f\u5927\u7814\u7a76\u751f\u548c\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u7684\u6b22\u8fce\uff0c\u6027\u4ef7\u6bd4\u7a81\u51fa\u3002<\/p>\n<h3>4. 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